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小鼠白介素11酶免試劑盒,(IL-11)ELISA檢測試劑盒,英文名:IL-11 ELISA Kit,英文縮寫:IL-11 ,常見試劑盒規(guī)格:96T/48T,產(chǎn)品別名:小鼠白介素11ELISA檢測試劑盒,IL-11 檢測試劑盒,IL-11 檢測試劑盒
ELISA實驗操作較繁雜,操作不當(dāng)將引起較大的誤差。小鼠白介素11酶免試劑盒,(IL-11)ELISA檢測試劑盒在操作中應(yīng)注意以下5個方面。
1.隨著病情的進(jìn)展或由其他因素影響,某些抗體在機體內(nèi)的含量發(fā)生大幅波動,因此有必要對標(biāo)本進(jìn)行預(yù)處理,例如對血清標(biāo)本作適當(dāng)稀釋,或離心分離血脂等。間接法測定中,標(biāo)本適當(dāng)稀釋還可降低非特異性反應(yīng)。
2.加樣一般使用加液器,在ELISA中一般有4次加樣:加標(biāo)本、加酶結(jié)合物、加底物和加終止液。加標(biāo)本時必須每份標(biāo)本使用一支移液器吸嘴,以免交叉污染。加酶結(jié)合物、底物和終止液時則不需更換吸嘴。
3.在成批手工檢測中,還應(yīng)注意操作時差的影響。加樣及混勻時間的差異,使抗原抗體反應(yīng)和酶促反應(yīng)時間不*,對競爭法及定量檢測影響很大,不注意可能會導(dǎo)致錯誤結(jié)果或定量不準(zhǔn)。
4.洗滌是ELISA操作過程中決定實驗成敗的一個關(guān)鍵步驟。目的是除去未結(jié)合的免疫反應(yīng)物,終止抗原抗體反應(yīng),除去標(biāo)本中與反應(yīng)無關(guān)的成分、游離的酶標(biāo)物以及反應(yīng)過程中吸附于固相載體上的非特異性物質(zhì)??捎孟窗鍣C洗板或手工洗板,前者洗滌質(zhì)量較好,各反應(yīng)孔洗滌效果均一,批內(nèi)、批間差異小,手工洗板應(yīng)注意控制各孔洗滌效果,差異不宜太大。
5.準(zhǔn)確判定結(jié)果須使用ELISA測讀儀(酶標(biāo)儀),比色法判讀可選擇單波長或雙波長,根據(jù)反應(yīng)液顏色的不同選用不同波長的濾光片,以空白孔校零測定反應(yīng)孔的吸光度值(A值)。酶標(biāo)儀具有良好的重復(fù)性。
組織型纖溶酶原激活因子(tPA)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Plasminogen Activator, Tissue (tPA)48T/96TY10826Rattus norvegicus (Rat)
組織型纖溶酶原激活因子(tPA)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Plasminogen Activator, Tissue (tPA)48T/96TY10827Mus musculus (Mouse)
免疫球蛋白G3(IgG3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Immunoglobulin G3 (IgG3)48T/96TY10828Homo sapiens (Human)
C反應(yīng)蛋白(CRP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for C Reactive Protein (CRP)48T/96TY10829Cavia (Guinea pig )
Ⅲ型膠原(COL3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Collagen Type III (COL3)48T/96TY10830Rattus norvegicus (Rat)
Ⅲ型膠原(COL3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Collagen Type III (COL3)48T/96TY10831Mus musculus (Mouse)
Ⅱ型前膠原氨基端原肽(PⅡNT)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Procollagen II N-Terminal Propeptide (PIINP)48T/96TY10832Homo sapiens (Human)
Ⅱ型前膠原氨基端原肽(PⅡNT)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Procollagen II N-Terminal Propeptide (PIINP)48T/96TY10833Rattus norvegicus (Rat)
Ⅱ型前膠原氨基端原肽(PⅡNT)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Procollagen II N-Terminal Propeptide (PIINP)48T/96TY10834Mus musculus (Mouse)
血小板激活因子(PAF)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Plaet Activating Factor (PAF)48T/96TY10835Homo sapiens (Human)
血小板激活因子(PAF)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Plaet Activating Factor (PAF)48T/96TY10836Rattus norvegicus (Rat)
氧化低密度脂蛋白(OxLDL)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Oxidized Low Density Lipoprotein (OxLDL)48T/96TY10837Homo sapiens (Human)
血小板衍生生長因子A(PDGFA)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Plaet Derived Growth Factor Subunit A (PDGFA)48T/96TY10838Homo sapiens (Human)
血小板衍生生長因子A(PDGFA)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Plaet Derived Growth Factor Subunit A (PDGFA)48T/96TY10839Mus musculus (Mouse)
血小板衍生生長因子A(PDGFA)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Plaet Derived Growth Factor Subunit A (PDGFA)48T/96TY10840Rattus norvegicus (Rat)
血栓調(diào)節(jié)蛋白(TM)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Thrombomodulin (TM)48T/96TY10841Homo sapiens (Human)
血栓調(diào)節(jié)蛋白(TM)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Thrombomodulin (TM)48T/96TY10842Mus musculus (Mouse)
血栓調(diào)節(jié)蛋白(TM)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Thrombomodulin (TM)48T/96TY10843Rattus norvegicus (Rat)
纖維蛋白原降解產(chǎn)物(FDP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Fibrinogen Degradation Product (FDP)48T/96TY10844Homo sapiens (Human)
纖維蛋白原降解產(chǎn)物(FDP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Fibrinogen Degradation Product (FDP)48T/96TY10845Mus musculus (Mouse)
纖維蛋白原降解產(chǎn)物(FDP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Fibrinogen Degradation Product (FDP)48T/96TY10846Rattus norvegicus (Rat)
纖溶酶原激活物抑制因子2(PAI2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Plasminogen Activator Inhibitor 2 (PAI2)48T/96TY10847Homo sapiens (Human)
纖溶酶原激活物抑制因子2(PAI2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Plasminogen Activator Inhibitor 2 (PAI2)48T/96TY10848Mus musculus (Mouse)
纖溶酶原激活物抑制因子2(PAI2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Plasminogen Activator Inhibitor 2 (PAI2)48T/96TY10849Rattus norvegicus (Rat)
纖溶酶原激活物抑制因子1(PAI1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Plasminogen Activator Inhibitor 1 (PAI1)48T/96TY10850Homo sapiens (Human)
纖溶酶原激活物抑制因子1(PAI1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Plasminogen Activator Inhibitor 1 (PAI1)48T/96TY10851Mus musculus (Mouse)
纖溶酶原激活物抑制因子1(PAI1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Plasminogen Activator Inhibitor 1 (PAI1)48T/96TY10852Rattus norvegicus (Rat)
細(xì)胞間粘附分子3(ICAM3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Intercellular Adhesion Molecule 3 (ICAM3)48T/96TY10853Homo sapiens (Human)
細(xì)胞間粘附分子2(ICAM2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Intercellular Adhesion Molecule 2 (ICAM2)48T/96TY10854Homo sapiens (Human)
細(xì)胞間粘附分子2(ICAM2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Intercellular Adhesion Molecule 2 (ICAM2)48T/96TY10855Mus musculus (Mouse)
細(xì)胞間粘附分子2(ICAM2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Intercellular Adhesion Molecule 2 (ICAM2)48T/96TY10856Rattus norvegicus (Rat)
神經(jīng)元特異性烯醇化酶(NSE)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Enolase, Neuron Specific (NSE)48T/96TY10857Homo sapiens (Human)
神經(jīng)元特異性烯醇化酶(NSE)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Enolase, Neuron Specific (NSE)48T/96TY10858Mus musculus (Mouse)
神經(jīng)元特異性烯醇化酶(NSE)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Enolase, Neuron Specific (NSE)48T/96TY10859Rattus norvegicus (Rat)
髓鞘堿性蛋白(MBP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Myelin Basic Protein (MBP)48T/96TY10860Sus scrofa; Porcine (Pig)
前列腺素E2(PGE2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Prostaglandin E2 (PGE2)48T/96TY10861General
腦鈉素(BNP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Brain Natriuretic Peptide (BNP)48T/96TY10862Homo sapiens (Human)
腦鈉素(BNP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Brain Natriuretic Peptide (BNP)48T/96TY10863Mus musculus (Mouse)
腦鈉素(BNP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Brain Natriuretic Peptide (BNP)48T/96TY10864Rattus norvegicus (Rat)
內(nèi)皮抑素(ES)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Endostatin (ES)48T/96TY10865Homo sapiens (Human)
內(nèi)皮抑素(ES)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Endostatin (ES)48T/96TY10866Mus musculus (Mouse)
免疫球蛋白M(IgM)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Immunoglobulin M (IgM)48T/96TY10867Homo sapiens (Human)
免疫球蛋白M(IgM)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Immunoglobulin M (IgM)48T/96TY10868Mus musculus (Mouse)
免疫球蛋白G(IgG)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Immunoglobulin G (IgG)48T/96TY10869Homo sapiens (Human)
免疫球蛋白G(IgG)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Immunoglobulin G (IgG)48T/96TY10870Mus musculus (Mouse)
免疫球蛋白G(IgG)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Immunoglobulin G (IgG)48T/96TY10871Rattus norvegicus (Rat)
免疫球蛋白E(IgE)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Immunoglobulin E (IgE)48T/96TY10872Mus musculus (Mouse)
免疫球蛋白E(IgE)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Immunoglobulin E (IgE)48T/96TY10873Rattus norvegicus (Rat)
免疫球蛋白A(IgA)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Immunoglobulin A (IgA)48T/96TY10874Homo sapiens (Human)
免疫球蛋白A(IgA)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Immunoglobulin A (IgA)48T/96TY10875Mus musculus (Mouse)
免疫球蛋白A(IgA)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Immunoglobulin A (IgA)48T/96TY10876Rattus norvegicus (Rat)
血管細(xì)胞粘附分子1(VCAM1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Vascular Cell Adhesion Molecule 1 (VCAM1)48T/96TY10877Homo sapiens (Human)
血管細(xì)胞粘附分子1(VCAM1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Vascular Cell Adhesion Molecule 1 (VCAM1)48T/96TY10878Mus musculus (Mouse)
血管細(xì)胞粘附分子1(VCAM1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Vascular Cell Adhesion Molecule 1 (VCAM1)48T/96TY10879Rattus norvegicus (Rat)
巨噬細(xì)胞炎性蛋白5(MIP5)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Macrophage Inflammatory Protein 5 (MIP5)48T/96TY10880Homo sapiens (Human)
巨噬細(xì)胞炎性蛋白相關(guān)蛋白1(MRP1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Macrophage Inflammatory Protein Related Protein 1 (MRP1)48T/96TY10881Mus musculus (Mouse)
巨噬細(xì)胞炎性蛋白5(MIP5)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Macrophage Inflammatory Protein 5 (MIP5)48T/96TY10882Rattus norvegicus (Rat)
堿性成纖維細(xì)胞生長因子(FGF2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Fibroblast Growth Factor 2, Basic (FGF2)48T/96TY10883Homo sapiens (Human)
堿性成纖維細(xì)胞生長因子(FGF2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Fibroblast Growth Factor 2, Basic (FGF2)48T/96TY10884Mus musculus (Mouse)
各種類型ELISA測定時,一般需經(jīng)過以下步驟:固相包被→加待測樣品→溫育→洗滌+加酶標(biāo)物→溫育→洗滌→加底物→溫育一加終止液→比色→判定結(jié)果。